See also a 2D gel of the TOM complex from Solanum tuberosum (potato)
Characterization of outer mitochondrial membrane proteins from Arabidopsis thaliana
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Two-dimensional resolution of outer mitochondrial
membrane proteins from Arabidopsis thaliana by Blue-native / Tricine
SDS polyacrylamide gel electrophoresis (Werhahn
et al. 2001, Plant Physiol. 125, 943-954, Werhahn
et al. 2003, Plant Pysiol. Biochem. 41, 407-416). One dominant protein
complex forms part of the outer mitochondrial membrane: "the preprotein
translocase of the outer mitochondrial membrane", also called the "TOM
complex". In Arabidopsis the TOM complex consists of 6 subunits
of 34 kDa (TOM40), 23/21 kDa (TOM20), 10 kDa (TOM22 or TOM9), 8 kDa (TOM7),
6.3 kDa (TOM6) and 6.0 kDa (TOM5). There are four different genes encoding
TOM20 in Arabidopsis, two genes encoding TOM40, TOM22 and TOM7 and single-copy
genes encoding TOM6 and TOM5.
See also a 2D gel of the TOM complex from Solanum tuberosum (potato) |
List of identified proteins
(last update: Jan. 2003)
Proteins were identified by Edman Degradation (ED) or
mass spectrometry (ESI-MS/MS, [MS]).
| Protein No | Identity | Accession | Identification | Sequences determined |
| 1 | TOM40 (34.1 kDa) | At3g20000 | ED | ADLLPPLTAAQVDAK |
| 2 | TOM20-2 (23.0 kDa) | At1g27390 | ED | KXPELHMQFMNQG... |
| 3 | TOM20-3 (22.6 kDa) | At3g27080 | ED | KHNFDLATQFFQQAVD... |
| 4 | TOM20-4 (21.0 kDa) | At5g40930 | ED | KASQFFQLAVEEQPES... |
| 5 | TOM9-2 (10.2 kDa) | At5g43970 | ED, MS | EAQINEIELQQASLLGA... |
| 6 | TOM7-1 ( 8.3 kDa) | At5g41685 | ED | KVNKGKGKGSKGASS... |
| 7 | TOM6 ( 6.3 kDa) | At1g49410 | ED | MFPGMFMRKPXK |
| 8 | TOM5 ( 6.0 kDa) | At5g08040 | ED | VNNVVSIEKMXA |