The Arabidopsis mitochondrial proteome project (1996-2004)

• 2D IEF/SDS PAGE
• 2D Blue-Native/SDS PAGE (Arabidopsis Mitochondria)
• 2D Blue-Native/SDS PAGE (Arabidopsis outer mitochondrial membrane)
• List of identified Proteins (Arabidopsis Mitochondria)
• 2D Blue-Native/SDS PAGE (Potato Mitochondria)
• 2D Blue-Native/SDS PAGE (Potato outer mitochondrial membrane)
• Publications

Two-dimensional resolution of the mitochondrial proteome from Arabidopsis thaliana by Blue-native / Tricine SDS polyacrylamide gel electrophoresis (Eubel et al. 2003, Plant Physiol. 133, 274-286). Arrows indicate identified proteins (a list of identified proteins is given below). Identities of the separated protein complexes (from left to right): Supercomplex of NADH dehydrogenase and dimeric cytochrome c reductase (~ 1500 kDa), dimeric F0F1 ATP synthase (~ 1100 kDa), NADH dehydrogenase (~ 1000 kDa), HSP60 complex (~ 750 kDa), F0F1 ATP synthase complex (~ 580 kDa), dimeric cytochrome c reductase (~ 480 kDa), cytochrome c oxidase (300 and 220 kDa) and succinat dehydrogenase (150 kDa). see also: Mitochondrial protein complexes from potato

Proteins were identified by Edman Degradation (ED) or mass spectrometry (ESI-MS/MS or MALDI-TOF-MS).
 
 

Protein No	Identity	Accession	Identification	Sequences determined
1	subunit of complex I	At1g67350	ESI	WDPQISQVAGR RDPYDDLLEDNYTPPSSSSSSSD
2	prohibitin	At5g40770	ESI	SPNVAYLPGGQSMLFALNR
3	8.0 kDa subunit, complex III	At3g52730	ESI	YEDISVLGQRPVEE
4	8.2 kDa subunit, complex III	At3g10860 At5g05370	ESI	AVVYALSPFQQK
5	6.7 kDa subunit, complex III	At3g15640	ESI	TFIDPPPTEEK
6	TOM40	At3g20000	ESI	IPTAHYEFGANYYDPK IDSNGVASALLEER
7	COX subunit 5b	At3g15640	ESI	RLDDIDFPEGPFGTK
8	flavoprotein subunit, complex II	At5g66760	MALDI	-
9	iron sulphur subunit, complex II	At5g40650	ESI	DLVVDMTNFYNQYK WNPDNPGKPELQDYKIDLK
10	iron sulphur subunit, complex II	At5g40650 At3g27380	ESI	DLVVDMTNFYNQYK
11	hyp. protein (complex II)	At1g47420	ESI MALDI ED	AAEAVEEFGGILTSIK YAEYLDSFEPEEVYLK SEDVSHMPEMDSXVLNAFK...
12a	hyp. protein (complex II)	At1g08480	MALDI ESI	FMEWWER LDTMAAQVK
12b	cytochrome c	At4g10040 At1g22840	ESI	QGPNLNGLFGR
13	subunit 3 of complex II	At5g09600	ED	STISGDIKTTQEEP
14	superoxide dismutase	At3g10920	ESI	LVVDTTANQDPLVTK YASEVYEKESN
15	g subunit, ccmplex V	At4g29480	ESI	QYIQEPATVEK LASIPGRYETFWK
16	COX subunit 2	y08501	ESI	LNQISILYQR