The Arabidopsis mitochondrial proteome project (1996-2004)

More recent data can be found at gelmap.de

An Arabidopsis thaliana mitochondrial proteome project was started for a comprehensive investigation of mitochondrial functions in plants. As a first step the proteome of mitochondria isolated from suspension cell cultures was characterized by high resolution 2D gel electrophoresis and mass spectrometry. Currently the proteomes of selected Arabidopsis knock-out mutants concerning nuclear genes of mitochondrial proteins are under investigation in our laboratory.
Holger Eubel and Hans-Peter Braun

The Arabidopsis mitochondrial proteome project


Two dimensional resolution of the mitochondrial proteome from Arabidopsis thaliana by IEF / Tricine SDS polyacrylamide gel electrophoresis (left side of the gel: pH 3, right side: pH 10, top: 100 kDa, bottom: 5 kDa). Futher informations are given in Kruft et al. (2001), Plant Physiol. 127, 1694-1710.

Arrows indicate identified proteins. Blue arrows indicate newly identified proteins (not given in Kruft et al. 2001).

List of identified proteins of the Arabidopsis mitochondrial proteome